The plasmid cloning vector pBR322 (see Fig. 9−4 ) is cleaved wi... | Filo
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Given below is the schematic diagram of a cloning vector pBR322... | Filo
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The plasmid cloning vector pBR322 (see Fig. 9-4 ) is cleaved with the restriction endonuclease P s t I. An isolated DNA fragment from a eukaryotic genome (also produced by PstI cleavage) is added to the prepared vector and ligated. The mixture of ligated DNAs is then used to transform bacteria, and plasmid-containing bacteria are selected by growth in the presence of tetracycline. (a) In addition to the desired recombinant plasmid, what other types of plasmids might be found among the transforme
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